Journal: bioRxiv
Article Title: GATA2 controls lymphatic endothelial cell junctional integrity and lymphovenous valve morphogenesis through miR-126
doi: 10.1101/660068
Figure Lengend Snippet: E12.0 Wild type and Lyve1-Cre;Gata2 f/f littermates or Tg(Prox1-tdTomato) and Tg(Prox1-tdTomato); Lyve1-Cre;Gata2 f/f littermates were frontally sectioned and IHC was performed for LVV-EC markers (PROX1, tdTomato, FOXC2, ITGA5, ITGA9), ECM components (FN1, LAMA5), and cell junction proteins (CD31, VE-Cadherin, GJA4, CLDN5). No obvious differences were observed between control and mutant samples. Abbreviations: A, artery; LS, lymph sac. Measuring bar: (A-P) 100 µm. Statistics: n=3 embryos and 6 LVV complexes per genotype per antibody.
Article Snippet: Primary antibodies for immunohistochemistry: rabbit anti-PROX1 (Cat# 11-002, Angiobio, San Diego, CA, USA), goat anti-human PROX1 (Cat# AF2727, R&D Systems, Minneapolis, MN, USA), sheep anti-mouse FOXC2 (Cat# AF6989, R&D Systems, Minneapolis, MN, USA), goat anti-mouse VEGRF3 (Cat# AF743, R&D Systems, Minneapolis, MN, USA), rat anti-mouse CD31 (Cat# 553370, BD Pharmingen, San Jose, CA, USA), goat anti-mouse ITGA9 (Cat # AF3827, R&D Systems, Minneapolis, MN, USA), rat anti-mouse VE-Cadherin (Cat# 550548, BD Pharmingen, San Jose, CA, USA), hamster anti-mouse PDPN (Cat# 127401, Biolegend, San Diego, CA, USA), rat anti-mouse ITGA5 (Cat #553319, BD Pharmingen, San Jose, CA, USA), goat anti-mouse GATA2 (Cat #AF2046, R&D Systems, Minneapolis, MN, USA), rabbit anti-mouse CX37 (Cat #40-4200, Life technologies, Grand Island, NY, USA), rabbit anti-mouse LAMA5 (Abcam, Cambridge, MA, USA), rabbit anti-human Fibronectin (Cat# ab2413, Abcam, Cambridge, MA, USA), Goat anti-human ANGPT2 (Cat# AF623, R&D Systems, Minneapolis, MN, USA), goat anti-mouse EGFL7 (Cat # AF3089, R&D Systems, Minneapolis, MN, USA), rabbit anti-mouse CLDN5 (Cat #34-1600, Thermo Fisher Scientific, Rockford, IL, USA), rabbit anti-mouse LYVE-1 (Cat # 11-034, Angiobio, San Diego, CA, USA).
Techniques: Mutagenesis
